The yin and yang of imaging tumor associated …

2019-10-15 · Iron oxide nanoparticles which can be directed by magnetic fields might be particularly useful for ferroptosis-based cancer therapy. Zhou et al. showed an iron oxide nanoparticle based delivery of linoleic acid hydroperoxide (LAHP) polymers to transport Fe 2+, generating ROS and 1 O 2 that has led to programmed cancer cell death or ferroptosis ...

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Metal Oxide Nanoparticles in Therapeutic …

2019-11-16 · Metal (zinc, iron, titanium, copper, etc.) oxide nanoparticles are widely used in therapeutic applications as drugs, nanocarriers, and diagnostic tools. Macrophages can recognize and engulf ...

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Iron Oxide Nanoparticles in Bioimaging – An Immune …

Iron oxide nanoparticles (IONPs) bear big hopes in nanomedicine due to their (potential) applications in tumor therapy, drug delivery or bioimaging. However, as foreign entities, such particles may be recognized by the immune system and, thus, lead to inflammation, hypersensitivity or anaphylactic shock. In addition, an overload with iron is known to cause …

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Iron oxide nanoparticles for immune cell labeling and …

Iron oxide nanoparticles (IONPs) are promising candidates for use in immunotherapy as they are biocompatible, have flexible surface chemistry, and display magnetic properties that may be used in contrast-enhanced magnetic resonance imaging (MRI). ... it is possible that they would elicit stronger immune response from macrophages. 88 To label ...

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US Patent for Iron oxide nanoparticles for use in treating …

2011-4-12 · Iron oxide nanoparticles are used to label and track inflammatory and stem cells by MRI [Leor J, Rozen L, Zuloff-Shani A, Feinberg M. S, Amsalem Y, Barbash I M, Kachel E, Holbova R, Mardor Y, Daniels D, Ocherashvilli A, Orenstein A, Danon D. Ex vivo activated human macrophages improve healing, remodeling, and function of the infarcted heart.

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Iron oxide nanoparticles inhibit tumour growth by …

Until now, the Food and Drug Administration (FDA)-approved iron supplement ferumoxytol and other iron oxide nanoparticles have been used for treating iron deficiency, as contrast agents for magnetic resonance imaging and as drug carriers. Here, …

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Mushroom Carboxymethylated β-d-Glucan Functions as a …

2022-6-2 · β-d-glucans have the potential of serving as both macrophage-targeted carriers and immune stimulators via inducing trained immunity in macrophages this study, a carboxymethylated β-glucan from mushroom sclerotium of Pleurotus tuber-regium (CMPTR) was combined with iron oxide nanoparticles (IONPs) to form nanocomplexes (CMPTR/IONPs) with …

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Targeted gold-coated iron oxide nanoparticles for CD163 …

2015-11-30 · Herein, we prepared gold-coated iron oxide nanoparticles functionalized with antibodies against CD163 as a T 2 contrast agent for the detection of M2-macrophages.

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Tracking stem cells and macrophages with gold and iron …

2019-6-1 · In this work, we optimized the conditions to label stem cells and macrophages with a library of gold and iron oxide NPs of different sizes ... In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells. Nanoscale Adv. (2019), 10.1039/C8NA00098K

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Functionalized superparamagnetic iron oxide nanoparticles p…

Mechanism of Iron Oxide-Induced Macrophage Activation: The

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Iron oxide nanoparticles inhibit tumour growth by …

2021-11-6 · Iron oxide nanoparticles have been widely used as contrast agents and drug carriers in preclinical and clinical settings 1–5.We recently revealed a hidden intrinsic therapeutic effect of the FDA-approved iron oxide nanoparticle compound ferumoxytol on ''untreated'' tumours: tumour cells co-injected with ferumoxytol exhibited a markedly delayed growth rate compared with …

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Uptake, distribution, clearance, and toxicity of iron oxide ...

2018-2-1 · Cell uptake of different IONPs in RAW264.7 macrophage cells and SKOV-3 cells. (A) Prussian blue staining of iron in RAW264.7 cells and SKOV-3 cells after incubation with SEI-10, SMG-10, and SMG-30 ...

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Iron oxide nanoparticles: synthesis, functionalization, and ...

2020-6-2 · In recent years, iron oxide nanoparticles have demonstrated great potential in biomedical applications due to their non-toxic role in biological systems. Also, the magnetic and semiconductor properties of iron oxide nanoparticles can lead to multifunctional applications in medicine. These nanoparticles have been developed as antibacterial, antifungal, and …

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Functionalized superparamagnetic iron oxide …

Functionalized superparamagnetic iron oxide nanoparticles provide highly efficient iron-labeling in macrophages for magnetic resonance-based detection in vivo ... An optimal labeling time of 4 h at 25 µg/mL was demonstrated to label macrophages of mouse and human origin without any adverse effects on cell viability whilst providing substantial ...

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Iron Oxide Nanoparticles as Theranostic Agents in Cancer …

2021-7-29 · In vitro and in vivo labeling of cells by using iron oxide NPs. (A) Experimental protocol adopted to label cells in vitro: NPs are added to the cell culture medium, if possible in the presence of transfection agents.After tests on cell viability and iron content, labeled cells are transplanted into living organisms and their in vivo homing can be visualized by MRI.

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Macrophage membrane-coated iron oxide nanoparticles …

2018-2-13 · Figure 1. Preparation of macrophage membrane-coated iron oxide nanoparticles (Fe 3 O 4 MM NPs) for enhanced cancer therapy. (A) Macrophage membrane-derived vesicles (MM-vesicles) were collected from macrophages and then coated onto Fe 3 O 4 NPs. The resulting Fe 3 O 4 MM NPs, which inherited (B) good biocompatibility, (C) immune evasion, (D) cancer …

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Ten Things You Might Not Know about Iron Oxide …

2017-8-21 · Iron Oxide Nanoparticles Do Not Localize to Macrophages in Lymph Nodes USPIOs have been used extensively for the detection of lymph node metastases ( 11, 12, 15 ). Previous studies showed that ferumoxtran-10 and ferumoxytol nanoparticles are retained in normal lymph nodes, causing a long-lasting T2 and T2* signal effect on T2- and T2 ...

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Differently Charged Super-Paramagnetic Iron Oxide …

Tumor-associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME); in some solid tumors, it counts for up to 50% of the cell population ( Komohara et al., 2014; Vitale et al., 2019 ). Macrophages could be simply classified as M1-like (pro-inflammatory) and M2-like (anti-inflammatory) phenotypes according ...

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Differently Charged Super-Paramagnetic Iron Oxide …

2020-5-29 · Macrophages are mainly divided into two phenotypes: M1-like (anti-tumoral, pro-inflammatory) and M2-like (pro-tumoral, anti-inflammatory). The more abundant M2-like phenotype of tumor associated macrophages (TAMs) has been associated with poor prognosis in various cancers, therefore, many studies have been carried out to modulate TAMs to change …

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Functionalized superparamagnetic iron oxide …

2017-2-15 · A series of cell health and iron uptake assays determined that positively charged SPIONs (+16.8 mV) could safely label macrophages more efficiently than the formerly approved ferumoxide (−6.7 mV; Endorem) and at least 10 times more efficiently than the clinically approved SPION ferumoxytol (−24.2 mV; Rienso).

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Nanoparticles'' toxic effects on macrophages

1  · Taken together, macrophage toxicity due to nanoparticles is mainly demonstrated through nanoparticle internalization, inflammatory responses, oxidative stress, cell death, and DNA damage. In the ...

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MRI coupled with clinically-applicable iron oxide …

2019-7-11 · Iron oxide magnetic nanoparticles highlight early involvement of the choroid plexus in central nervous system inflammation. ASN Neuro 5 …

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Iron oxide nanoparticles oxidize transformed RAW 264.7 …

1  · 1. Introduction. Iron oxide nanoparticles (IONPs) were ubiquitous existed in atmospheric particulate matter that may generate from traffic emission and iron ore mining (Gonet and Maher, 2019).IONPs can also be emitted from coal combustion, melting of engine fragments (Gminski et al., 2011; Maher et al., 2020).Thus, IONPs exposure caused widely public concerns for their …

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Mechanism of Iron Oxide-Induced Macrophage Activation: …

2019-4-17 · Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1). ... Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway J Am Chem Soc. 2019 Apr 17;141(15 ...

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Fluorophore-Conjugated Iron Oxide Nanoparticle Labeling …

2009-1-2 · The use of fluorophore-labeled iron oxide nanoparticles for fluorescence imaging in combination with flow cytometry allows evaluation of labeling efficiencies and homing capabilities of defined human HSC subsets. ... such as macrophages. To overcome this limitation, a number of approaches have been described to modify the dextran coating to ...

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Functionalized superparamagnetic iron oxide …

2017-4-1 · To determine the optimal parameters required for iron labeling in macrophages, we designed, synthesized and tested three dextran particles as described in Table I.The three particles had a neutral surface charge (Dex), a positive surface charge (DEAE-Dex 4:1) or a negative surface charge (CM-Dex) and were incubated with BMDMs for 24 h over a range of …

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In Vitro Model of Bromodeoxyuridine or Iron Oxide …

2008-2-14 · Quantifying the percentage of macrophages that incorporate the iron oxide nanoparticles or BrdU label from viable, apoptotic, or dead cells will aid in the interpretation of the results of vivo cellular imaging and microscopic tracking analysis. M aterials and M ethods Cell Culture and Labeling with FE-Pro and BrdU

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Iron oxide nanoparticles promote macrophage autophagy …

2019-5-1 · Superparamagnetic iron oxide nanoparticles (SPIONs) are excellent MRI contrast agents used for imaging of cancer, cardiovascular and inflammatory diseases in both research and clinical applications. The surface coating material of SPIONs is a major factor determining their in vivo distribution and biosafety. Resovist (ferucarbotran) and ...

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Immunological Effects of Iron Oxide Nanoparticles and …

1. Introduction. Nanotechnology gained much attention during the past decade due to nanoparticles'' physicochemical properties that allow investigators to cover gaps and overcome barriers in various industrial and biomedical applications [1–3].One such barrier is related to the delivery of therapeutic and imaging agents [].Nanoparticles help to address this by providing …

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Iron oxide nanoparticles promote macrophage autophagy …

2019-2-1 · Yes LC3 -- [124] Iron [140] Lanthanum oxide 15-186 nm THP-1 Yes LC3, SQSTM1 -LAMP1 [141] Ytterbium oxide 177 nm HeLa Yes LC3 -Lysotracker [142] Europium oxide ≈50 nm HeLa Yes LC3 -Lysotracker ...

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Iron oxide nanoparticle-induced hematopoietic and …

1. Introduction Iron oxide nanoparticles (IONPs) attracts great scientific interest in biomedical fields due to high bio-availability, generation of heat in alternating magnetic field, and localization into specific tissue under the influence of an external magnetic field. 1,2 Due to this unique property, IONPs hold immense potential in biomedical applications such as MRI contrast agent ...

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Poly (dopamine) coated superparamagnetic iron oxide …

2016-1-5 · The superparamagnetic iron oxide nanoparticles (SPIONs) clusters were obtained as previous description 24. Briefly, 1.2 mL of hydrophobic SPIONs nanoparticles (100 mg/mL in toluene) obtained by ...

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The Use of Iron Oxide Nanoparticles to Reprogram …

2021-6-9 · The synthesis and functionalization of iron oxide nanoparticles (IONPs) is versatile, which has enhanced the interest in studying them as theranostic agents over recent years. ... The Use of Iron Oxide Nanoparticles to Reprogram Macrophage Responses and the Immunological Tumor Microenvironment Front Immunol. 2021 Jun 9;12:693709. doi: 10.3389 ...

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Imaging macrophages with nanoparticles | Nature Materials

2014-1-23 · c, MRI of macrophages using iron oxide nanoparticles (ATHEROMA trial) 138 and gadolinium-containing high-density lipoprotein (Gd-HDL) particles 137. The arrowheads indicate atherosclerotic plaque.

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Iron Oxide Magnetic Nanoparticles: Characterization and …

The aim of this study was to evaluate the biological properties of iron oxide nanoparticles (IO-NPs) obtained in the aqueous suspension. The iron oxide nanoparticles were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The biocompatibility of the iron oxide was demonstrated by the in vitro > quantification of HeLa …

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Iron oxide nanoparticles promote macrophage autophagy …

In this study, we demonstrated that two clinically used superparamagnetic iron oxide nanoparticles (SPIONs) specifically induced macrophage autophagy through activation of TLR4, followed by phosphorylation of p38 and nucleus translocation of Nrf2, leading to upregulation of p62/SQSTM1 and macrophage scavenger receptor SR-AI mRNA expression.

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Macrophage endocytosis of superparamagnetic iron oxide …

Rationale and objectives: Superparamagnetic iron oxides (SPIO) used as magnetic resonance (MR) contrast agents undergo specific uptake by macrophages. The purpose of this study was first to determine the mechanism of macrophage uptake for Ferumoxides by using competition experiments with specific ligands of scavenger receptors SR-A (I/II) and second, to evaluate …

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